U87 Xenograft Model - Altogen Labs
U87 xenograft is a widely used model of human glioblastoma multiforme (GBM), a highly aggressive and malignant form of brain cancer. The U87 xenograft model is created by injecting human U87MG glioblastoma cells into immunodeficient mice, which allows the cancer cells to grow and form tumors that closely resemble human GBMs.
Imaging of Fibroblast Activation Protein in Cancer Xenografts …
2021年4月8日 · Mouse xenograft models were developed with FAP-positive U87 and FAP-negative PC3 cells to test pharmacokinetics and binding specificity in vivo. QCP01 and [111 In]QCP02 demonstrated nanomolar inhibition of FAP at K i values of 1.26 and 16.20 nM, respectively. Both were selective for FAP over DPP-IV, a related serine protease.
Real-time imaging of glioblastoma using bioluminescence in a U-87 MG ...
2015年2月4日 · Here, we generated xenograft model mouse of GBM in which U-87 MG human glioblastoma cells were intracranially implanted for live imaging. Introducing luciferase gene into U-87 MG cell line enabled real-time observation and quantification of tumor survival and propagation by detecting photon emission derived from luciferase.
Imaging of Fibroblast Activation Protein Alpha Expression in a ...
2020年8月12日 · Clearance and retention properties of [89 Zr]Zr-Df-Bz-F19 were investigated through biodistribution studies using a xenotransplantation model consisting of nude mice bearing FAP + U87MG tumors.
A New Systemic Disease Mouse Model for Glioblastoma Capable …
2024年1月19日 · Our novel mouse model extended on this finding, using the existing genetic mutations of our xenograft cell lines to investigate tumour progression. Expanding on our findings, we propose that glioblastoma recurrence is preordained at the time of diagnosis whereby glioblastoma tumour cells have already infiltrated systemically throughout the brain.
Development of ibuprofen-modified fibroblast activation protein ...
4 天之前 · Nude mice bearing A549-FAP or U87MG tumors were injected with a dose of 0.74−1.5 MBq/mouse and evaluated at 1, 4, 24, 72, and 168 h post- injection. The specificity of the radiotracers was investigated by co-injecting a subset of mice with 177 Lu-labeling FAP ligands and the competitor DOTA-FAPI-04 at a dose of 57 nmol/mouse, with analysis ...
Cancer cell-mitochondria hybrid membrane coated Gboxin loaded ...
2023年7月28日 · Effective tumour inhibition in orthotopic U87MG GBM and patient derived X01 GBM stem cell xenografts in female mice with extended survival time and negligible side effects are also noted. We...
Secreted protein acidic and rich in cysteine mediates active targeting ...
To demonstrate the mechanism of HSA accumulation in tumors, we injected FNR648-labeled HSA and FITC-labeled dextran in U87MG and U87MG-shSPARC tumor-bearing mice and observed their micro-distribution in tumor tissues. Results: HSA was internalized in cells by binding with SPARC in vitro.
Patient-derived xenografts (PDX) provide in vivo glioblastoma (GBM) models that recapitulate actual tumors. Orthotopic tumor xenografts within the mouse brain are obtained by injection of GBM stem-like cells derived from fresh surgical specimens. These xenografts reproduce GBM’s histologic complexity and
Hallmarks of tumor-associated microglia response to experimental …
2021年10月1日 · Microglia in U87 GBM xenograft showed similar pattern as in human glioblastomas. Microglia infiltration closely correlated with tumor volume, proliferation and vessel density in U87 GBM xenograft. This U87 GBM xenograft represents a good model for further testing microglia as a target and new strategy to fight with.