The prefered medium of Bacillus subtilis is LB medium. The growth follows the typical four phases of bacterial growth (figure 2) and with reaching the exponential growth phase, Bacillus subtilis has a doubling time of 30 minutes under ideal conditions. This can be calculated with the slope of the exponential growth phase. In our calculation,
Optimization of fermentation conditions for surfactin production by B …
Apr 26, 2023 · The standard curve of surfactin was made according to the peak area of the liquid phase and the concentration of the standard as shown in Fig. 2 b. The equation of the standard curve was y = 3,805,784,50x + 280,003.58, and the correlation coefficient R 2 was 0.9997, demonstrating a good correlation.
B. subtilis (B246), originally isolated from the avocado phylloplane (Korsten et al., 1988), was selected for optimizing mass cell production due to its strong inhibitory action against economically important avocado post-harvest pathogens. The antagonists were maintained on standard 1 nutrient agar (STD) (Biolab) slants and
Optimization of Growth Conditions for the Production of Bacillus ...
Jan 10, 2022 · Glucose was used as the standard for carbohydrate analysis and was prepared at different concentrations in the tubes to form the standard curve. Analysis was followed by the addition of 5 mL of H 2 SO 4 .
Standard curve of the Bacillus subtilis cell population derived …
Standard curve of the Bacillus subtilis cell population derived from the plating count compared with that from qPCR. The X-axis represents the number of strain NCD-2 cells that inoculated...
Growth curve of B. Subtilis. | Download Scientific Diagram
Figure 1 shows the growth curve of the test organism marked by different phases such as lag phase, log phase, and stationary phase. The bacteria concentrations required for addition in the...
Developing rapid growing Bacillus subtilis for improved …
Dec 1, 2020 · Specific growth rate of engineered B. subtilis increased 53.06% compared with B. subtilis 168, reaching 0.75 h-1 in M9 medium. Production of RNA, acetoin, and ovalbumin increased 38.09%, 5.40%, and 19.79% using fast-growing strain as chassis.
Statistics and simulation of growth of single bacterial cells ...
Nov 23, 2017 · Here we validate and exploit existing theory on the statistics of single-cell growth in order to predict the probability of phenotypic characteristics such as cell-cycle times, volumes, accuracy of...
An efficient transformation method for Bacillus subtilis DB104
Mar 7, 2012 · Growth curve of B. subtilis DB104 in SM1 medium (filled squares) and SM1/SM2 medium (open squares). Optical density (OD 600) monitored at 600 nm in function of time. The cells were grown (37 °C, 200 rpm) and 1-ml aliquots …
Qualitative and Quantitative Analyses of the Colonization ...
Apr 8, 2020 · Standard curve of the Bacillus subtilis cell population derived from the plating count compared with that from qPCR. The X-axis represents the number of strain NCD-2 cells that inoculated into the soil. The Y-axis represents the Ct value of strain NCD-2 detected by qPCR