Visualization of trigeminal ganglion neuronal activities in mice
Because the trigeminal ganglion (TG) is located deep within the cranial cavity of mice, few studies have been able to access to it. To circumvent the limitation, overlying brain tissue must to be removed to expose the TG, so that optical recording can access deep brain neural ensembles.
Human and mouse trigeminal ganglia cell atlas implicates
2022年6月1日 · We thus developed a cell atlas in which human and mouse trigeminal ganglia are transcriptionally and epigenomically profiled at single-cell resolution. These data describe evolutionarily conserved and human-specific gene expression patterns within each trigeminal ganglion cell type, as well as the transcription factors and gene regulatory ...
Visualization of Trigeminal Ganglion Neuronal Activities in Mice
This unit describes a procedure for conducting non-survival surgery to visualize the TG in live mice. Obtaining large ensembles of direct, real-time readouts of sensory neuron signaling, providing temporal and spatial information across the TG, will help to define the cellular basis of orofacial somatic sensing and pain perception. © 2019 by ...
Trigeminal ganglion neurons are directly activated by influx of …
2024年7月4日 · CSF collected from animals exposed to CSD activates trigeminal neurons in naïve mice in part by CSF-borne calcitonin gene–related peptide (CGRP). We identify a communication pathway between the central and peripheral nervous system that might explain the relationship between migrainous aura and headache.
Dissecting Mouse Trigeminal Ganglia: A Comprehensive Analysis …
Purpose : The trigeminal ganglion (TG) plays a pivotal role in eye diseases like neurotrophic and herpes keratitis. Despite the development of multiple mouse models to explore TG physiology and pathology, the understanding of mouse TG anatomy remains unclear, which has impacted interpretations of many datasets.
Human and mouse trigeminal ganglia cell atlas implicates …
2022年6月1日 · Yang et al. developed a cell atlas in which human and mouse trigeminal ganglia are transcriptionally and epigenomically profiled at single-cell resolution. These data provide a rich resource for understanding the cell types and genetic mechanisms underlying migraine susceptibility and for developing novel analgesics.
Mapping the face in the somatosensory brainstem | Nature …
2010年2月24日 · Positional patterning of trigeminal ganglion primary sensory neurons sets the stage for the somatotopy of ophthalmic, maxillary and mandibular central afferents, transforming the spatial...
Transcriptional Alterations of Mouse Trigeminal Ganglion …
2022年6月1日 · Orofacial inflammation leads to transcriptional alterations in trigeminal ganglion (TG) neurons. However, diverse alterations and regulatory mechanisms following orofacial inflammatory pain in different types of TG neurons remain unclear. Here, orofacial inflammation was induced by injection of complete Freund’s adjuvant (CFA) in mice.
Cross-talk signaling in the trigeminal ganglion: role of ... - Springer
2020年2月22日 · Using transgenic mice with GFP expression in only sensory cells, neurons in the DRG and trigeminal ganglion were separated from non-neuronal cell types by FACS and analyzed by RNAseq (Lopes et al. 2017). The two different sensory ganglia had almost identical gene expression with the exception of 63 genes.
Harmonized cross-species cell atlases of trigeminal and dorsal root ganglia
2024年6月21日 · Sensory neurons in the dorsal root ganglion (DRG) and trigeminal ganglion (TG) are specialized to detect and transduce diverse environmental stimuli to the central nervous system. Single-cell RNA sequencing has provided insights into the diversity of sensory ganglia cell types in rodents, nonhuman primates, and humans, but it remains difficult ...