Molecular probes for tracking lipid droplet membrane dynamics
2024年10月31日 · In this work, we present a three-pronged molecular probe design strategy that combines lipophilicity-based organelle targeting with microenvironment-dependent activation and design an LD...
Fluorescent Probes for Lipid Membranes: From the Cell Surface …
2022年12月19日 · Membrane probes for biophysical sensing are based on environment-sensitive fluorophores: (1) polarity-sensitive solvatochromic dyes; (2) viscosity-sensitive fluorescent molecular rotors; (3) mechanosensitive fluorescent flippers; and (4) voltage-sensitive electrochromic dyes.
Fluorescent Probes for Lipid Rafts: From Model Membranes to …
2014年1月16日 · We highlight an urgent need to develop new probes, specifically adapted for cell plasma membranes and compatible with modern fluorescence microscopy techniques to push the understanding of membrane microdomains forward.
A fluorescent membrane tension probe | Nature Chemistry
2018年8月27日 · Here we show that a planarizable push–pull fluorescent probe called FliptR (fluorescent lipid tension reporter) can monitor changes in membrane tension by changing its fluorescence lifetime...
Fluorescent Probes for Lipid Rafts: From Model Membranes to …
Membrane microdomains (rafts) remain one of the controversial issues in biophysics. Klymchenko and Kreder review the current state in the field of fluorescent molecular probes, which make these lipid nanostructures visible, and highlight an urgent need to develop new probes to study membranes in living cells.
Probes for Lipids and Membranes—Chapter 13 - Thermo Fisher Scientific
Introduction to Membrane Probes—Section 13.1. Fluorescent and Biotinylated Membrane Probes; Other Probes for Studying Cell Membranes
Fluorescent probes for the imaging of lipid droplets in live cells
2021年1月15日 · Each probe displayed excellent biocompatibility and was found to be sufficiently lipophilic to allow for the effective imaging of LDs in HeLa cells. These Pyr probes were successfully applied to the study of lipid metabolism in live zebrafish.
Single-Molecule Probes of Lipid Membrane Structure
2008年11月16日 · Probing membrane organization, structure, and changes therein are crucial for linking structural metrics with function in biological membranes. Here we report the use of single-molecule fluorescence studies to measure membrane structure at the molecular level.
Tutorial: fluorescence lifetime microscopy of membrane ... - Nature
2024年8月29日 · In this Tutorial, we cover the development of hydrophobic mechanosensing fluorescent probes called Flippers, whose fluorescence lifetime depends on lipid packing. Flipper probes can therefore...
Monitoring Lipid Droplet Dynamics in Living Cells by Using …
2019年1月10日 · These Lipi-probes are LD-specific probes that contain a pyrene or perylene group as a fluorescent scaffold and can be used to observe dynamics of LD in live cells and also interrelations with other organelles by simultaneous staining …
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