Fast phage detection and quantification: An optical density
2019年5月9日 · In this work we carry out a detailed study calibrating optical density kinetics as a function of both, phage abundance and concentration of the host organisms. Our study determines the percentage of growth inhibition from integrated growth curves and correlates this value to the amount of phage initially present in the sample.
Centroid of the bacterial growth curves: a metric to assess phage ...
2024年5月31日 · Phage replication can be studied using various approaches, including measuring the optical density (OD) of a bacterial culture in a liquid medium in the presence of phages. A few...
Isolation, characterization, and antibacterial activity of lytic ...
Adsorption (A) and one-step growth curve of phage in MRSA ATCC 6538 (B). After 5 min, the phage exhibited rapid adsorption to the bacteria. The one-step growth curve indicated a latent period of 30 min and a burst time of 70 min, corresponding to …
Centroid of the bacterial growth curves: a metric to assess phage ...
2024年5月31日 · Phage replication can be studied using various approaches, including measuring the optical density (OD) of a bacterial culture in a liquid medium in the presence of phages. A few quantitative methods are available to measure and compare the efficiency of phages by using a single index based on the analysis of OD curves.
Clinical Phage Microbiology: a suggested framework and recommendations ...
2021年8月16日 · Growth curves of different Pseudomonas aeruginosa isolates in logarithmic (A) and stationary (B) phases of growth. Different anti- P aeruginosa bacteriophages were added to the cultures, grown in 96-well plates, and repeated …
Isolation, screening and characterization of phage - ScienceDirect
2023年1月1日 · Bacterial growth curves were obtained by plotting OD after baseline adjustment against time; these curves provide a quantitative measure of growth responses that facilitate direct comparisons between control growth (untreated) and phage-treated bacteria at …
PhageScore: A simple method for comparative evaluation of ...
2020年9月15日 · In this study, two main parameters were set: (i) a ≥2 log10 decrease in bacterial numbers after 8 and 20 h (i.e. measurement of bactericidal efficacy), and (ii) a time of ≥480 min to reach an OD 420–580 of 0.1 above the original OD 420–580 (a measurement of bacterial re-growth post phage infection).
Escherichia coli phage phi2013: genomic analysis and receptor ...
2022年10月4日 · The data showed that phage phi2013 can efficiently inhibit E. coli growth in the tested foods at 4°C and 25°C. We therefore conclude that phage phi2013 or cocktails containing phi2013 may be used as an antimicrobial agent in extending the shelf-life of food products by effectively controlling the growth of E. coli.
Growth inhibition curve of phage phi2013. MOI, multiplicity of...
Here, we report the isolation and characterization of the lytic virus phi2013, which is specific for Escherichia coli laboratory strains. Transmission electron microscopy showed that phage...
Practical Methods for Determining Phage Growth Parameters
In this chapter we present protocols for determining phage growth parameters, particularly phage rate of adsorption, latent period, eclipse period, and burst size. Bacteriophagy always takes place in the same manner; the sequence of events is always the same.