DNA–DNA hybridization - Wikipedia
In genomics, DNA–DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between DNA sequences. It is used to determine the genetic distance between two organisms and has been used extensively in phylogeny and taxonomy .
Hybridization - National Human Genome Research Institute
3 天之前 · Hybridization, as related to genomics, is the process in which two complementary single-stranded DNA and/or RNA molecules bond together to form a double-stranded molecule. The bonding is dependent on the appropriate base …
DNA-DNA Hybridization - an overview | ScienceDirect Topics
DNA hybridization involves the formation of a double-stranded nucleic acid, either a DNA double helix or an RNA–DNA duplex. Typically this involves use of a single-stranded radioactive probe sequence, prepared from all or part of a cloned gene, or a chemically synthesized oligonucleotide.
8.7: DNA Analysis- Blotting and Hybridization
2023年6月20日 · Genomic DNA that has been digested with a restriction enzyme is separated on an agarose gel, then the DNA is transferred from the gel to a nylon membrane (grey sheet) by blotting. The DNA is immobilized on the membrane, then probed with a radioactively labeled DNA fragment that is complementary to a target sequence.
Kinetics and Dynamics of DNA Hybridization
2011年6月30日 · In this Account, we examine recent studies of the kinetics and dynamics of DNA hybridization, including (i) intramolecular collision of random coil, single-stranded DNA (ssDNA), (ii) nucleic acid hairpin folding, and (iii) considerations of DNA hybridization from both a global view and a detailed base-by-base view.
DNA Hybridization - an overview | ScienceDirect Topics
DNA hybridization may be used to assess the genetic similarity between two populations of DNA or to detect a specific sequence of DNA in a population of DNA molecules. This is achieved by using a nucleic acid probe.
Predicting DNA hybridization kinetics from sequence
2017年11月6日 · Next-generation sequencing (NGS) studies show a significant correlation (R 2 ≈ 0.6) between the rate constants of DNA hybridization in single-plex versus multiplex, suggesting that the current ...
Dna Hybridization - Encyclopedia.com
2018年6月11日 · In the molecular genetic technique of hybridization of DNA, single strands of DNA from two different species are allowed to join together to form hybrid double helices. These hybrid segments of DNA can be used to determine the evolutionary relatedness of organisms by examining how similar or dissimilar the DNA base pair sequences are.
Nucleic Acid Hybridization. A Theoretical Consideration
DNA-DNA and RNA-DNA hybridization reactions are the basis of many assays in DNA analysis and are presently some of the most frequently used techniques in molecular biology. The hybridization reaction is the formation of partial or complete double-stranded nucleic acid molecules by sequence-specific interaction of two complementary single ...
DNA Hybridization - an overview | ScienceDirect Topics
DNA hybridization involves the formation of a double-stranded nucleic acid, either a DNA double helix or an RNA–DNA duplex. Typically this involves use of a single-stranded radioactive probe sequence, prepared from all or part of a cloned gene, or a chemically synthesized oligonucleotide.