Programming bacteria for multiplexed DNA detection
2023年4月10日 · Here we develop cell-based DNA sensors by engineering the naturally competent bacterium Bacillus subtilis (B. subtilis) to detect specific DNA sequences in the environment. The DNA sensor...
DNA detection using recombination proteins - PubMed
Our novel approach, recombinase polymerase amplification (RPA), couples isothermal recombinase-driven primer targeting of template material with strand-displacement DNA synthesis. It achieves exponential amplification with no need for pretreatment of sample DNA. Reactions are sensitive, specific, and rapid and operate at constant low temperature.
Detection of Nucleic Acids and Proteins - The Cell - NCBI Bookshelf
Detection of DNA by nucleic acid hybridization. A specific sequence can be detected in total cell DNA by hybridization with a radiolabeled DNA probe. The DNA is denatured by heating to 95°C, yielding single-stranded molecules.
Point‐of‐Care Testing (POCT) Devices for DNA Detection: A …
DNA chips play a crucial role in point-of-care diagnostics by enabling rapid and accurate detection of genetic information. These chips offer high sensitivity and selectivity, allowing for the identification of specific DNA sequences associated with diseases and pathogens.
Electric field-enhanced electrochemical CRISPR biosensor for DNA detection
2021年11月15日 · DNA detection plays an important role in the rapid screening of cancers and early diagnosis of infectious diseases. Here, we developed a simple, versatile, electric field-enhanced (EFE), electrochemical CRISPR biosensor …
Highly multiplexed rapid DNA detection with single-nucleotide ...
2021年7月1日 · Here we show that a portable and battery-powered PCR assay performed in a toroidal convection chamber housing a microarray of fluorescently quenched oligonucleotide probes allows for the rapid and...
CDetection: CRISPR-Cas12b-based DNA detection with sub …
2019年7月1日 · CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher ...
DNA Detection Using Recombination Proteins | PLOS Biology
2006年6月13日 · Our novel approach, recombinase polymerase amplification (RPA), couples isothermal recombinase-driven primer targeting of template material with strand-displacement DNA synthesis. It achieves exponential amplification with no need for pretreatment of sample DNA. Reactions are sensitive, specific, and rapid and operate at constant low temperature.
DNA Diagnostics: Nanotechnology-Enhanced Electrochemical Detection of ...
Detection speed: the detection of DNA involves several processes including DNA recognition, sample manipulation, and signal readout. By using EC sensors, DNA recognition can occur within...
DNA Detection Using a Single-Layer Phosphorene Nanopore
2023年4月26日 · By conducting hundreds of molecular dynamics simulations, we evaluate the phosphorene nanopore’s sensitivity to distinguish between different nucleotides through the generation of characteristic features such as fluctuations in ionic current, residence time, and the dehydration state of the nanopore.