Apoptosis Analysis by Western Blotting - Bio-Rad
Analysis of the middle phase of extrinsic apoptosis by western blotting can be achieved by detection of the effector caspase-3 and caspase-7 and in particular the active forms of caspase-3 and caspase-7.
Determination of Caspase Activation by Western Blot - PubMed
In the context of apoptosis, the proper analysis of western blot results depends on the understanding of the mechanisms and outcomes of caspase processing during the course of its activation. In this chapter, we describe the step-by-step methodology in the western blot analysis of caspase cleavage during apoptosis.
The prodomain of caspase-3 regulates its own removal and caspase …
2019年1月28日 · We directly assessed caspase-3 activation following serum starvation by measuring caspase-3 cleavage via western blot analysis. When caspase-3 is not cleaved it has a molecular weight of...
Caspase-3(CASP3)靶点介绍及实验小贴士| Abcam中文官网
Caspase-3是一种半胱氨酸-天冬氨酸蛋白酶，在细胞凋亡过程中起到关键作用，被广泛用作凋亡的生物标志物。 Caspase-3在凋亡过程中，被剪切成分子量分别为17kDa（p17）和12kDa（p12）的两个片段，并易位至细胞核。 Caspase-3经过剪切激活后，可以激活 caspase-6、-7 和 -9，形成凋亡级联反应。 Caspase-3参与了亨廷顿蛋白的降解。 Caspase-3参与了淀粉样蛋白的前体蛋白切割，与阿尔茨海默病中神经元的死亡有关。 在肺，脾，心脏，肝脏和肾脏中高度表达。 脑和骨 …
Caspase-3 Antibody - Cell Signaling Technology
Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa). The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST.
Detecting Cleaved Caspase-3 in Apoptotic Cells by Flow Cytometry
These fragments are most commonly detected by western blotting, which resolves proteins and their fragments based on molecular mass. However, antibodies that only recognize cleaved fragments can be used to specifically label cells in which caspase cleavage has occurred.
Prospective clinical biomarkers of caspase-mediated apoptosis ...
2017年7月18日 · Clinical and experimental data indicate that caspase-3-mediated apoptosis plays a key role in cleavage of tau, an initial process underlying formation of fibrillary tangles and amyloid plaques that are commonly observed in AD and other neurodegenerative disorders[106,112,119,120,121,122] and two the most recognized hallmarks of ...
Determination of Caspase Activation by Western Blot
2020年12月15日 · This chapter describes the step-by-step procedure required for the detection of caspase activation by western blot or immunoblot technique. The steps are broken down into five parts: (1) induction of apoptosis, (2) protein extraction, (3) protein quantitation, (4) polyacrylamide gel electrophoresis, and (5) western blot analysis.
Determination of Caspase Activation by Western Blot
In this chapter, we describe the step-by-step methodology in the western blot analysis of caspase cleavage during apoptosis. We detail protocols for protein extraction, quantitation, casting, and running gel electrophoresis and western blot analysis of caspase -8 and caspase -9 activation.
Immunodetection of caspase-3 by Western blot using …
2011年8月15日 · Because Western blot processing of membranes may lead to protein loss, we tested whether the glutaraldehyde fixation of blotted membranes could improve sensitivity of caspase-3 immunodetection. We resolved recombinant activated caspase-3 by 15% SDS–PAGE and transferred to a NC membrane, and the blotted membrane was incubated with different ...