MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell …
MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.
MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS
MitoSOX is among the most commonly used probe for detecting cellular ROS. MitoSOX accumulates in mitochondria due to its positive charge, and MitoSOX-based assays, especially flow cytometry, have been frequently used to detect mitochondrial ROS formation in cells under various conditions.
MitoSOXTM Red mitochondrial superoxide indicator is a novel fluorogenic dye for highly selective detection of super-oxide (Figure 1) in the mitochondria of live cells. MitoSOXTM Red reagent is live-cell permeant and is rapidly and selec-tively targeted to the mitochondria.
MitoSOX Red | Mitochondrial ROS Indicator - MedChemExpress
MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems.
MitoSOXTM Green (MSG) reagent and MitoSOXTM Red (MSR) reagent are live-cell permeant which rapidly and selectively target the mitochondria. Once in the mitochondria, the MSG reagent or MSR reagent are oxidized by superoxide and exhibit bright green or …
Oxidative Stress Detection | Thermo Fisher Scientific - US
New MitoSOX Green Mitochondrial Superoxide Detection Reagent. Visualize mitochondrial superoxide in your live cells with specificity and ease-of-use, MitoSOX Green offers: Highly selective for mitochondrial superoxide, not other ROS/NOS; Easy imaging with a standard FITC/GFP fluorescence filter on any fluorescence microscope
MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS
In this ROS Protocols article, we described the flow cytometric protocol involving the use of various concentrations of MitoSOX (1, 2.5, 5 μM) for detecting mitochondrial ROS in control and mitochondrial DNA-deficient (MD) melanoma B16-F10 cells.
A Closer Look at Mitochondrial Dysfunction With MitoSOX …
Many of the applications of MitoSOX™ Red indicator for specific detection of mitochondrial superoxide in live cells (Figure 1) and tissues discussed in this article revolve around SOD and Complex I, disorders in their expression, structure, and function, and their ultimate consequences in pathologies including amyotrophic lateral sclerosis ...
High-content fluorescence imaging with the metabolic flux
2020年5月29日 · MitoSOX is a mitochondrially targeted fluorescent dye 13 that is oxidized specifically by superoxide to ethidium. The ethidium then intercalates into mitochondrial DNA and thus produces ...
Live Cell Imaging of Mitochondrial Redox State in Mammalian …
We describe use dyhydroethidium (DHE) and MitoSOX (a derivative of dihydroethidium bound to a lipophilic cation) to detect mitochondrial superoxide in yeast and mammalian cells, respectively. We also describe use of genetically encoded fluorescent biosensors for quantitative analysis of mitochondrial NADPH levels (iNap) in mammalian cells and ...