Citrate Utilization Test- Principle, Media, Procedure and Result
2022年8月10日 · Principle of Citrate Utilization Test. Citrate agar is used to test an organism’s ability to utilize citrate as a source of energy. The medium contains citrate as the sole carbon source and inorganic ammonium salts (NH4H2PO4) as the sole source of nitrogen.
The citrate test screens a bacterial isolate for the ability to utilize citrate as its carbon and energy source (3, 7). A positive diagnostic test rests on the generation of alkaline by-products of citrate metabolism. The subsequent increase in the pH of the medium is demonstrated by the color change of a pH indicator.
Citrate Utilization Test- Principle, Procedure, Results, Uses
2022年1月10日 · What is Citrate Utilization Test? The citrate utilization test is a part of the IMViC test (Indole, Methyl Red, Vogues-Proskauer, and Citrate Test) that differentiates organisms on the basis of their ability to use citrate as a sole source of energy.
1.20: Citrate Test - Biology LibreTexts
Explain what the citrate test indicates about a bacteria species' genes, enzymes, and metabolism. Tell what citrate permease is and what it does for bacterial species that have it. Tell that the citrate test is useful for bacterial identification and characterization. Successfully conduct a citrate test and interpret the results.
Citrate Utilization Test: Principle, Procedure, Results
A citrate utilization test is used to determine the ability of bacteria to utilize sodium citrate as its only carbon source and inorganic ammonium dihydrogen phosphate (NH4H2PO4) as the sole nitrogen source.
Citrate Test Protocol
2009年12月1日 · The citrate test screens a bacterial isolate for the ability to utilize citrate as its carbon and energy source. A positive diagnostic test rests on the generation of alkaline by-products of citrate metabolism. The subsequent increase in the pH of the medium is demonstrated by the color change of a pH indicator.
Citrate Test in Microbiology: Pathways, Metabolism, and …
Explore the citrate test in microbiology, focusing on its metabolic role, utilization pathways, and result interpretation techniques. Microbiologists often rely on various biochemical tests to identify and differentiate between bacterial species.
Citrate Test in Bacterial Identification: Principle and Results
2024年7月5日 · In industrial microbiology, the citrate test is employed to monitor and control microbial contamination in food and pharmaceutical production. Certain bacteria can spoil products or pose health risks, making it essential to identify and …
Simmons Citrate Agar- Composition, Principle, Preparation, …
2022年1月5日 · Simmons citrate agar is a selective and differential medium that tests for an organism’s ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source. It is used for differentiating gram-negative bacteria on the basis of citrate utilization.
Citrate Test - Principle, Biochemistry, Media, Procedure, Result ...
2022年8月15日 · The citrate utilization test is a biochemical test used to determine if the test organism is able to metabolize citrate as the sole of carbon and energy with resulting alkalinity. For the citrate utilization test, Simmon's citrate agar is used. It contains sodium citrate as the only source of carbon and energy and indicator bromothymol blue.