Sequencing and cloning of antigen-specific antibodies from mouse …
Here we describe a protocol to sequence and clone the variable antibody region of single antigen-specific mouse memory B cells for antibody production. After purification, antigen-specific …
A simplified workflow for monoclonal antibody sequencing
2019年6月24日 · We successfully sequenced the variable regions of five mouse monoclonal IgG antibodies using this method, which enabled us to design chimeric mouse/human antibody …
Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies …
2021年6月14日 · We applied the method to sequence the widely used anti-FLAG-M2 mouse monoclonal antibody, which we successfully validated by remodeling a high-resolution crystal …
Sequencing and cloning of antigen-specific antibodies from mouse memory ...
2016年9月15日 · Here we describe a protocol to sequence and clone the variable antibody region of single antigen-specific mouse memory B cells for antibody production. After purification, …
Sequencing, cloning, and antigen binding analysis of monoclonal ...
2021年6月6日 · Here, we describe a protocol for the sequencing, cloning, and characterization of antibody genes that encode BCRs. We used this method to analyze the BCRs of different …
Hybridoma antibody cloning and sequencing protocols
2016年5月23日 · The first method established to use PCR to amplify and clone mouse immunoglobulin (Ig) variable (V) regions after selective first-strand cDNA synthesis. The …
Efficient amplification and direct sequencing of mouse variable …
1999年6月11日 · This strategy could be a powerful tool for antibody sequence assessment whatever the V gene family before humanization of mouse monoclonal antibody or …
Large-scale network analysis reveals the sequence space ... - Nature
2019年3月21日 · Although antibody sequence diversity varied highly among mice (74–85% unique clones in a given mouse, Supplementary Fig. 2a), we found a remarkable cross-mouse …
Mouse Antibody - labome.com
2022年11月20日 · The mouse is the predominant animal host for monoclonal antibody production and also a host for polyclonal antibody generation. While typical mouse monoclonal antibody …
NAb-seq: an accurate, rapid, and cost-effective method for antibody ...
2022年8月13日 · Here, we leveraged recent improvements in long-read Oxford Nanopore Technologies (ONT) sequencing to develop Nanopore Antibody sequencing (NAb-seq): a …