If the particles are visible in the optical microscope, the area can be marked with a "Sharpie" pen. The pen marks can be seen in the FIB image. Otherwise the particles will have to be located in the ...

Enhanced FIB-SEM images of untreated cells and cells treated with Torin, which causes nutrient stress. The ER exit sites (magenta), ER (blue), and all lysosome-related organelles (cyan ...

The Hitachi FB-2000A FIB uses a beam of focused high-energy (30 kV) gallium ions to remove material in a very controlled manner from inorganic specimens. The FB-2000A is a single beam system; that is, ...

Here, a platinum electrode measuring one hundredth of a nanometer has been deposited on the tip of this pyramid shaped AFM tip via focused ion beam (FIB) deposition. (Image: C. Menozzi, G.C. Gazzadi, ...