3D render of Human Embryonic Kidney cells (HEK-293 cell line) spreading in viscous medium. Viscous medium was added at 16:30. Colour-coded for height, where cooler colours are higher. Video ...

In this approach, researchers can simply transfect their preferred host cell—in most cases, human embryonic kidney (HEK) 293 cells—with all three plasmids in a stepwise procedure (see Figure 1).

We obtained LDLs of 42 and 1,624 cells for the A431 and HEK-293 cell lines, demonstrating assay sensitivity. The relative expression of EGFR in the two cell lines was in agreement with published ...