"The effort of producing high-resolution cryo-EM images is wasted if they are compromised by poor sample preparation," Usukura says. He advises that a good sample for cryo-EM is, most importantly ...

such as cryo-electron microscopy (cryo-EM) single-particle averaging (SPA) analysis, rely on averaging data from thousands of ...

This work will be of high interest to a broad swathe of scientists studying native macromolecules present at low concentrations in cells. Cryo-EM single-particle analyses typically require target ...

For the first time, researchers at the University of California San Diego report near-atomic-resolution images and video of nitrogenase during catalysis using cryogenic electron microscopy (cryoEM).

This “exposure” yields many 2-D images of the molecules at different angles, which algorithms then merge into one 3-D model. At first cryo-EM rendered molecules as vague lumps, so some ...

Researchers performing cryo-EM experiments can acquire the training to make their own cryo-EM and negative-stain grids, and collect and process their own high-resolution data. We also offer sample ...

“Cryo-EM allows us to peer into a world that we simply ... Subsequently, a 3D map of the target protein is created using ...

This was because the resulting images were little more than blobs - hence the rude description of "blobology" by its detractors. But over time, scientists refined cryo-EM to the point that it ...

Purdue Uni Cryo-EM was used to determine the structure of ... Over a few months, 3-D images of the virus at atomic resolution were generated and researchers could start searching for potential ...