This “exposure” yields many 2-D images of the molecules at different angles, which algorithms then merge into one 3-D model. At first cryo-EM rendered molecules as vague lumps, so some ...

This work will be of high interest to a broad swathe of scientists studying native macromolecules present at low concentrations in cells. Cryo-EM single-particle analyses typically require target ...

In a groundbreaking development, researchers from the United States and Denmark have successfully captured 3D images of ...

Moreover, data collection at low temperature reduces sample damage by the electron beam, improving the resolution of the EM images. This paper thus marks the early development of cryo-EM.

For the first time, researchers at the University of California San Diego report near-atomic-resolution images and video of nitrogenase during catalysis using cryogenic electron microscopy (cryoEM).