Anion-exchange chromatography is commonly used for final purification of plasmid DNA 13 ... were subjected to alkaline lysis, as in the large-scale separation. Cell debris was removed by ...

can be pelleted by centrifugation leaving the cell extract as a reasonably clear supernatant. 2. Purification ... containing Lysis Solution T/Proteinase K. Incubate at 55 °C until the tissue is ...

Biotage® PhyPrep is the first automated solution for plasmid purification in Maxi, Mega and Giga scales. The various scales differ in the volume of cell culture to be purified, with desired yields ...