I had to find the right field of view where the differentiated and non-differentiated cells interacted.This took about three hours of precise observation under the microscope to capture the right ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

Emission signal from this volume is collected by a CCD camera positioned perpendicular to this plane – imaging the entire XY dimension as you would in a traditional epifluorescence microscope. The ...