The pen marks can be seen in the FIB image. Otherwise the particles will have to be located in the FIB using the M0–50 beam. Some form of a protective layer may be required as described in the ...
Enhanced FIB-SEM images of untreated cells and cells treated with Torin, which causes nutrient stress. The ER exit sites (magenta), ER (blue), and all lysosome-related organelles (cyan ...
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