(a) The basic elements of FISH are a DNA probe and a target sequence. (b) Before hybridization, the DNA probe is labeled by various means, such as nick translation, random primed labeling ...
The CyBio FeliX, a benchtop instrument for automated liquid handling tasks, was used to automate the HaloPlex Target ...
All steps of ISH – from pre-hybridization to additions of probes, hybridization, stringency washes and antibody-mediated chromogenic or fluorescent reaction – are carried out by the robotic system.
In its basic form (i.e., hybridization, stringent washing ... should perform serial probing of an immobilized test DNA with allelic probes for both variants. By including an intervening alkali ...
One example is a technique called fluorescence in situ hybridization (FISH), which involves applying DNA probes to a chromosome spread, which are then read by a cytogeneticist. To use FISH ...